Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis
نویسندگان
چکیده
منابع مشابه
A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient
Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...
متن کاملComparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes
BACKGROUND Toxoplasmosis is an infectious disease caused by the parasitic protozoan Toxoplasma gondii. It is endemic worldwide and, depending on the geographic location, 15 to 85% of the human population are asymptomatically infected. Routine diagnosis is based on serology. The parasite has emerged as a major opportunistic pathogen for immunocompromised patients, in whom it can cause life-threa...
متن کاملUsefulness of real-time PCR assay targeting lipL32 gene for diagnosis of human leptospirosis in Uruguay.
INTRODUCTION Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. METHODOLOGY We analyzed by microscopic agglutination test (MAT) and lipL32 qPCR serum samples...
متن کاملa real time pcr assay on blood for diagnosis of invasive candidiasis in immunocompromised patient
background and purpose: invasive candidiasis (ic) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent ic progress is necessary. we established a real-time pcr assay on blood for the diagnosis an...
متن کاملSimulation of between Repeat Variability in Real Time PCR Reactions
While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process. Besides influences of more obvious factors such as camera noise and pipetting variation, changing efficiencies within and between reactions affect PCR results to a degree ...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2006
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2005.01332.x